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Viability assay

TP Theresa Pesch
HS Harald Schuhwerk
PW Philippe Wyrsch
TI Timo Immel
WD Wilhelm Dirks
AB Alexander Bürkle
TH Thomas Huhn
SB Sascha Beneke
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Cells were seeded into 96well-plates (clear bottom/white walls, Corning) and grown for 24 h. Medium was exchanged against medium containing toxins and incubated for 24 h or 48 h. Supernatant was replaced with fresh medium containing 9 μg/ml resazurin (Sigma) and incubated until medium in control wells turned purple. Fluorescence (excitation/emission 560 nm/590 nm) was measured using LS55-spectrometer (Perkin-Elmer). Each experiment was performed in technical duplicates or triplicates.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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