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The Bombyx mori non-diapausal strains (D9L) was provided from the Gene Resource Library of Domesticated Silkworm, Southwest University in China. The larvae were reared on fresh mulberry leaves at 25 ± 2 °C under a photoperiod of 12 h light/12 h dark with 75% relative humidity. Further, testis and ovary samples from the fifth instar larval stage were collected for isolation of total RNA and protein for different experimental analyses.
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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