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2.5. Luciferase reporter assay
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For the luciferase reporter assay, cells were seeded into 24‐well plates. The cells were cotransfected with WT‐STAT3 promoter‐PGL3 and mutated STAT3 promoter‐PGL3 luciferase constructs and a Renilla plasmid into the indicated cells overnight. Then, luciferase activity was analyzed with a Dual‐Luciferase Reporter Assay System after 24 hours (Promega, CA) according to the manufacturer's instructions.
Copyright and License information: ©2019 The Authors. Published by Wiley Periodicals, Inc.
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