To prepare plant extract, leaves and raw fruits were first surface sterilized with distilled water followed by 70% ethanol (Merck Ltd) for removal of dust and unwanted particles. Seeds were obtained from raw fruits by removing the pulp and sterilized with 70% ethanol (Merck, Ltd). Sterilized plant material (leaves and seeds) were sun-dried and then powdered with mechanical grinder. For extraction, soxhlet method was opted, were methanol (Fisher Scientific, Ltd) served as a solvent. For each plant, different conditions such as ratio of solvent (ml): plant material (g) and time were optimized. Extracted samples were further concentrated using Rota-evaporator (Rotavac Heidolph).
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