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Measurement of local SDF-1α levels in injured tissues
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A total of 96 rats were divided randomly into four equal groups: sham, and 1, 3, and 6 s electrical burns. At 0, 1, 3, and 7 days after injury, six rats in each group were sacrificed to collect samples. Tissue homogenates were prepared and centrifuged at 10,000 ×g at 4˚C for 20 min to collect supernatants. SDF-1α concentrations in the supernatants were determined according to the instructions of the SDF-1α ELISA kit.
Copyright and License information: ©2018 He et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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