A total of 2,000 full-length, non-redundant influenza B HA sequences were downloaded from the NCBI FLU database 55. All 2,000 sequences were aligned and analyzed using BIOEDIT software. In this analysis, a residue was considered conserved only if it was the same between two sequences. The values reported for percent conservation are the number of sequences with an identical residue at a position divided by the total number of sequences.
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