Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Glycerol in media was measured using Free Glycerol Reagent (Sigma Aldrich, St Louis, MO, USA) and Amplex UltraRed (Invitrogen), according to the manufacturers’ instructions. Amplex Ultra Red was diluted 100-fold in Free Glycerol Reagent, mixed with 20 μl of conditioned medium in a 96-well plate, and incubated at room temperature for 15 min. Fluorescence was measured (excitation/emission wavelengths 530 nm / 590 nm) using an Infinite M200 plate reader (Tecan Group, Männedorf, Switzerland).
Copyright and License information: The Author(s) ©2018
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
