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Acute lung injury model and measurement of wet-to-dry (W/D) ratio of lung weight
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The acute lung injury model was established in male rats by LPS administration (5 mg/kg, intravenous injection) (Shen et al. 2009) and the rats were sacrificed 6 h after LPS injection. The lungs were collected quickly and the left lung was separated. The wet weight of the left lung was measured after simple handling with filter paper. The left lung was then dried at 60 °C for 48 h and the dry weight of the lung was measured to calculate the W/D ratio.
Copyright and License information: ©2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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