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LCMV Clone 13 virus was produced by infecting BHK-21 cells with an LCMV Clone 13 stock at an MOI of 0.01 and harvesting viral supernatants 48 hours later. Viral titers were determined by plating diluted viral stocks on Vero cells with an agarose overlay. Four days later the Vero cells were stained with neutral red dye, and plaques quantified 14 hours later.
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