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TEM Analysis

AO Agata Olszewska-Widdrat
GS Gabriele Schiro
VR Victoria E. Reichel
DF Damien Faivre
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For electron microscopy, M. magneticum cells were concentrated by centrifugation (5000 rpm, 5 min, 4°C), adsorbed onto a cooper grid, rinsed once with buffer (10 mM Hepes, 5 mM EDTA) and once with distilled water. The samples were imaged using a Zeiss 912 Omega transmission electron microscope at 120 kV.

The transmission electron microscopy images were analyzed with “ImageJ” in order to determine the size of magnetosomes, their amount per cell and the cell length (Supplementary Figures S2B–F). The magnetosomes dimensions were estimated by calculating the best fit of ellipse to the contours (Devouard et al., 1998).

Copyright and License information:  ©2019 Olszewska-Widdrat, Schiro, Reichel and Faivre.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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