Sensitivity Tests to Different Fungicides

Mycelial plugs were taken from the colony margin and placed on the center of PDA plates amended with fludioxonil (0, 0.00625, 0.0125, 0.025, 0.05, and 0.1 μg/ml) or procymidone (0, 0.0625, 0.125, 0.25, 0.5, and 1 μg/ml) or iprodione (0, 0.125, 0.25, 0.5, 1, and 2 μg/ml). Three replicates per concentration for each strain. After incubation for 4 days at 25 C, the colony diameter was measured perpendicularly. EC₅₀ (the effective concentration for 50% inhibition of mycelial growth) was calculated by the regression of the probit of the percentage of inhibition of radial growth against the logarithmic value of fungicide concentration. The experiments were repeated three times independently.