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Serum triglyceride (TG) level was measured using the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI), total cholesterol, low density lipoprotein cholesterol (LDL), and high-density lipoprotein cholesterol (HDL) levels were determined using Cholesterol Assay Kit - HDL and LDL/VLDL (Abcam, Cambridge, MA). Serum levels of adiponectin (Abcam) and C-reactive protein (CRP; Abcam) were measured using commercially available rat ELISAs. Assays were conducted as described in the protocol provided by the manufacturer.
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