Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Scanning electron microscopy (SEM)

NM NULL Mamat-Noorhidayah
KY Kenjiro Yazawa
KN Keiji Numata
YN Y. Norma-Rashid
request Request a Protocol
ask Ask a question
Favorite

After imaging by laser scanning confocal microscopy, the forewings were coated with gold-palladium and examined using a JOEL JCM-6000 NeoScope Benchtop scanning electron microscopy (SEM) at accelerating voltage of 5kV. The samples were mounted using carbon tape and examined for the presence of cuticular spikes in close proximity to the vein joint on the dorsal and ventral. Subsequently, a detailed joint-by-joint mapping of the spikes was done.

Copyright and License information:  ©2018 Mamat-Noorhidayah et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A