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Effect of freeze-drying on thermo-responsive properties of Pluronic F127/HA solution
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The freeze-dried samples were prepared by immerging a polytetrafluoroethylene tube containing PF127/HA solution into liquid nitrogen for several minutes to let them freeze, and then the frozen sample was lyophilized by a freeze drier (Christ Alpha 4.2 LD over, Germany) at -40 °C and a pressure of 0.4 bar for about 48 h. To study the thermo-responsive behavior, the powder of the dried polymer was first equilibrated in deionized water at 4 °C, and then temperature was increased to 40 and 50 °C.
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