Peptides

The peptides were purchased from GenScript (Piscataway, USA). Peptide concentration was determined by the bicinchoninic acid assay as described by the supplier (Sigma) with Bovine Serum Albumin as a standard. 25 μL of each sample were mixed with 200 μL of a solution of bicinchoninic acid and copper sulfate 50:1 (v/v). The preparation was incubated at 37°C for 30 minutes and the absorbance at 595 nm was measured by a microtiter plate reader OPSYS MR (ThermoLabsystems).