The E. coli network constructed by Trevino [6] and the EcoliNet gene-prediction tool [10] were used to compare candidate gene lists. For direct comparison, we overlapped the modules with the same biological function. Genes with unknown function in Darkred module were submitted to EcoliNet [10] for prediction.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.