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The assayed proteins were diluted in protein buffer to the required concentrations and incubated at room temperature for 20 min. Loading dye (50% (w/v) glycerol, 0.2% (w/v) bromophenol blue, 300 mM DTT) was added before loading the samples on a 10% native acrylamide gel. Gels were run on ice at 150 V in 25 mM Tris–HCl, 250 mM glycine buffer and proteins were visualized by Coomassie staining.
Copyright and License information: ©2019 The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
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