2.6. Determination of drug encapsulation efficiency

The lipid bilayer of drug loaded liposomes was disrupted with methanol and sonicated for 10 min followed centrifugation at 13000 rpm for 15 min and the released material is then quantified by fluorescence spectroscopy. EGCG, Quercetin and Doxorubicin were excited at 325, 365 and 480 nm with fluorescence emission maxima recorded at 458, 463 and 530 nm respectively. Percentage of drug encapsulation efficiency and drug loading efficiency was calculated as follows:

Where T is the total amount of drug in the supernatant and sediment, and C is the amount of drug in the supernatant [18].