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Western Blot analysis and fluorescence assays
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Western Blot analyses of GFP and FLAG fusion proteins followed previously published protocols (38). Signals were visualized using a Fusion FX EDGE imager (Vilber) and band intensities were quantified using the BIO-1D software (Vilber). Fluorescence assay of V. cholerae and E. coli strains were performed as previously described (22,37).
Copyright and License information: ©2019-01-15 The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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