In-vitro Shoot Regeneration

Cotyledons obtained from 3-week-old seedlings were used as explants for shoot regeneration. The cotyledons were first excised from the seedlings and cut at both ends. The cotyledons were then cultured on solid full-strength MS medium supplemented with 6-benzylaminopurine (BAP) and Kinetin. Kinetin (0.5, 1.0, 1.5, 2.0 mg/L) and BAP (1.0, 2.0, 3.0, 4.0 mg/L) were used singly or in combinations and maintained in the culture room under 16 hours light/8 hours dark photoperiod at 24°±1°C. The explants were sub-cultured every three weeks. The percentage of callus, shoot induction and the average number of shoots induced per explant were observed and recorded after six weeks of culture.