The irradiated rectus femoris skin tissues were fixed with 4% paraformaldehyde overnight, paraffin‐embedded and sliced into sections of 4 m. The sections were dewaxed with xylene, rehydrated with graded concentrations of ethanol and then stained with haematoxylin and eosin reagents (Solarbio, G1120). An inverted microscope (Zeiss, Axio Scope A1) was used for observation.
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