Cells (5000/well) were seeded in 96-well plates for 72 h. Then cells were treated with 10 µM harmine. According to the manufacturer’s instructions, we assessed the cell viability with an MTS assay kit (Promega, Madison, WI). The absorbance value of the live cells residing in the 96-well plates was measured at 515 nm on a microplate reader (Thermo Fisher).
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