Analysis of ChIP-seq data

HF Hector L. Franco
AN Anusha Nagari
VM Venkat S. Malladi
WL Wenqian Li
YX Yuanxin Xi
DR Dana Richardson
KA Kendra L. Allton
KT Kaori Tanaka
JL Jing Li
SM Shino Murakami
KK Khandan Keyomarsi
MB Mark T. Bedford
XS Xiaobing Shi
WL Wei Li
MB Michelle C. Barton
SD Sharon Y.R. Dent
WK W. Lee Kraus
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The raw reads were aligned to the human reference genome using default parameters in Bowtie (ver. 1.0.0) (Langmead et al. 2009). The aligned reads were subsequently filtered for quality and uniquely mappable reads using SAMtools (version 0.1.19) (Li et al. 2009) and Picard (version 1.127; http://broadinstitute.github.io/picard/). Relaxed peaks were called using MACS (v2.1.0) (Feng et al. 2012) with a P-value = 1 × 10−2. Additional details about quality control and trimming, read alignment, and peak calling are provided in the Supplemental Methods.

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