Establishment of cell lines stably expressing or knocking down PCAT1

LH Lijie Huang
YW Yan Wang
JC Jiao Chen
YW Yu Wang
YZ Yabing Zhao
YW Yali Wang
YM Yunping Ma
XC Xin Chen
WL Wenzhong Liu
ZL Zhengzheng Li
LZ Lianmei Zhao
BS Baoen Shan
XD Xin Dong
DL Dan Li
SS Shujuan Shao
YS Yongmei Song
QZ Qimin Zhan
XL Xuefeng Liu
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To obtain cell lines stably expressing PCAT1, KYSE150 and KYSE450 cells were transfected with the pcDNA3.1 control plasmid or pcDNA3.1-PCAT1 plasmid using Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer’s instructions and then selected with G418 (300 μg/mL) for 2 weeks. To generate cell lines with stable knockdown of PCAT1, KYSE30 cells were transfected with the pGPH1/Neo control plasmid (sh-NC) or PCAT1-sh-1/sh-2 plasmid using Lipofectamine 2000 and selected with G418 (300 μg/mL) for 2 weeks. The cell lines with stable overexpression or knockdown of PCAT1 were identified using RT-qPCR.

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