2.8. Bioinformatic analysis

Quantitative Insights into Microbial Ecology (QIIME) pipeline (v.1.8.0; Morris et al., 1989) was used for the analysis of sequencing data. The statistical analysis was done with R. The reads that did not match with the specific sample barcode were discarded. In addition read shorter or longer than 50 pm and 480 bp, respectively, were also discarded. Further, OTUs with 97% similarly were formed by UCLUST (Edgar, 2010). PyNAST was used to align both representative and reference sequences (Caporaso et al., 2010). Further, taxonomic assignments and relationships between microbial communities were quantified.