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Infection of Mouse Macrophage Cell Line J774A.1 with BCG and BCG-MSP-1C cells
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After culturing for overnight, the mouse macrophage cell line J774A.1 (5 × 105 cells/mL) were infected with the BCG or BCG-MSP1C cells (1 × 107 cfu/ml) at a multiplicity of infection (MOI) of 1:20 in complete DMEM at 37°C in the presence of 5% CO2 for 48 h. Cells treated with LPS (100 ng/ml) were used as a positive control while untreated cells were used as a negative control.
Copyright and License information: Penerbit Universiti Sains Malaysia, ©2018
This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/).
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