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Genomic DNA methylation using SssI methylase enzyme

MA Milad Ahani-Nahayati
SS Saeed Solali
KA Karim Shams Asenjan
AA Ali Akbar Movassaghpour Akbari
MT Mehdi Talebi
MH Milad Zadi Heydarabad
SB Sina Baharaghdam
MH Majid Farshdousti Hagh
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Methylated DNA needed to be prepared as the positive control for MSP. DNA was extracted from peripheral blood and methylated with the Sss1 methylase enzyme (Biolabs, New England, US) following producer’s directions (16). The methylated DNA was immediately extracted via YT9040 DNA extraction kit and exposed to SBS treatment, then used as a positive control for the methylated DNA-specific primer (meth primer).

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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