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Sperm analysis

SR Siti Eliana Rochmi
MS Miyayu Soneta Sofyan
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Sperm concentration was estimated by a spectrophotometry. Sperm motility was assessed as a percentage of progressive sperm motility (forward direction movement). A drop of diluted semen was deposited on a clean glass slide and examined under 400× and then subjectively assessed. Evaluation of spermatozoa motility was done every 24 h until sperm motility decreased to at least 40%. Sperm motility was observed under 100× (for observing the motility of mass spermatozoa) and 400× (for observing the motility of individual spermatozoa). Percent viability was calculated via differential staining using eosin–nigrosin [14]. Slides were used for estimating the percentage of abnormal sperm on the basis of observable abnormalities. A minimum of 200 sperms were counted on each slide for the calculation of live and dead sperms as well as to note the number of abnormal sperms per sample.

Copyright and License information:  ©2019 Rochmi and Sofyan.
Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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