Cobas 4800 BRAF V600 Test kit

The Cobas 4800 BRAF V600 Mutation Test kit (Roche Molecular Diagnostics, Pleasanton, CA, USA) was used to detect the V600 mutation by real-time polymerase chain reaction. A set concentration of DNA was analyzed on the Cobas^® z 480 analyzer (Roche Molecular Diagnostics) according to the manufacturer's protocol. All runs and specimen validation were performed using the Cobas z 480 software (version 2.0; Roche Molecular Diagnostics). The results were generated automatically as a report using the Cobas z 480 software.