In vitro data (AC‐SINS and FcRn affinity)

AC‐SINS and FcRn affinity data were generated for all mAbs in the data set (mAbs 1–31). The AC‐SINS assay has been described in the literature.11, 13 In brief, mAbs are captured by anti‐human Fc antibodies coated on the gold nanoparticles. If a mAb tends to interact with itself, there is a clustering of the nanoparticles that leads to a red shift in the absorbance wavelength. This assay was used as a surrogate for non‐specific CL. The methodology for FcRn affinity measurement was described by Avery et al.11