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RNA immunoprecipitation (RIP) was performed using thermo fisher RIP kit (Thermo, U.S.A.) based on the manufacturer’s protocol. The Ago2 antibodies are purchased from Abcam (U.S.A.). Purified RNA was subjected to qRT-PCR analysis to demonstrate the presence of the binding targets using respective primers.
Copyright and License information: ©2019 The Author(s).
This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
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