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RNA from liver and skeletal muscle was extracted using Qiagen RNeasy Mini kit and reverse transcribed using RT2 first strand kit according to manufacturer's protocol. Qiagen microarray fatty acid metabolism (PAMM‐007Z) and fatty liver (PAMM‐157Z) were used and were analyzed using RT2 Profiler PCR Array Data Analysis (http://saweb2.sabiosciences.com/pcr/arrayanalysis.php). Automatic selection from Housekeeping group panel was used as a method of normalization.
Copyright and License information: ©2019 The Authors. published by Wiley Periodicals, Inc on behalf of American Neurological Association.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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