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CCA cells were counted and seeded in 6-well plates with 500 cells/well. After treatment with 25 μM Tm for 24 hours, the Tm was removed and normal medium was added for 14 days. The cells were fixed in methanol and colonies were counted after staining with 0.1% crystal violet in 25% methanol for 20 mins. The results were presented as the average number of counted colonies per well under each condition. All the experiments were performed in triplicate and repeated three times independently.
Copyright and License information: ©2019 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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