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Stimulation with dexamethasone (Dex)
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Dexamethasone (Sigma; code: D4902) was solved in pure ethanol and diluted into concentrations of 1 mM, 10 mM, 100 mM and 1000 mM. For exposure the Dex solutions were applied 1:1000 in 1 % FBS in DMEM (2 μl Dex + 2 ml 1 % FBS in DMEM). Thus the final concentrations of Dex were 1 nM, 10 nM, 100 nM and 1000 nM. The durations of stimulation (time points) were 6 h, 12 h, 24 h, 48 h, 72 h, 96 h and 120 h. The control, unstimulated cells, were exposed to pure ethanol using the same volume (2 μl ethanol + 2 ml 1 % FBS in DMEM).
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