Measurement of climbing efficiency in flies

Newly eclosed flies (1 day old) were fed with SY diet containing ellagic acid for 21 days. This long-term treatment is given to make the flies adapted to ellagic acid. Flies were transferred to empty tubes marked at 15 cm height. The tubes were tapped thrice to gather the flies at the bottom. Time taken by flies to climb the 15-cm mark was noted down using stopwatch. The assay was repeated three times with an interval of 30 min between each repetition. For each treatment and sex along with their respective control, twenty vials containing 10 flies/vial were counted. The total number of flies used was 1200. Data were subjected to two-way ANOVA followed by Bonferroni’s multiple comparisons test in the GraphPad Prism software v.6