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Cells were plated in 24 well plates and were stained with 0.1% crystal violet solution. Cell viability was detected using the crystal violet staining protocol, solubilization of the dye adsorbed by cell nuclei during staining of drug- and DMSO-treated cells was performed after 48 h of treatment and quantified using spectrophotometry.
Copyright and License information: ©2017 The Authors. Published by Elsevier Inc. on behalf of Neoplasia Press, Inc.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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