Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Construction of doxycycline-inducible cell lines
Request a Protocol
Ask a
question
Favorite
T-REx 293 cell lines inducibly expressing protein with doxycycline were established as described previously (12,39). To express a protein, 100 ng/ml of doxycycline was treated to the cells for the indicated time periods in the corresponding figure legends.
Copyright and License information: ©2019 The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
