2.5. Histopathology scoring

Histopathologist (J.B.Fu.), blinded to experimental conditions, used H&E-stained sections to evaluate the degree of inflammation at each electrode site (E1–E4). Histological changes were on a scale of 0–3 for the assessment of the extent and the degree of damage to the mucosa, and on a scale of 0–2 for assessment of the numbers of leucocytes within venules and the extent of haemorrhage within villi [26]. Scores were out of a total of 10 (table 1).

Histological scoring table: parameters used to score inflammation on a scale from 0 to 10.