Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Tumor xenograft in NOD-SCID mice
Ask a
question
Favorite
The cells were cultured in a low-cell adhesion plate for 7 days followed by LCSC ball collection. Cells were washed with normal saline, triturated gently and prepared into a single cell suspension, followed by cell counting. A total of 2 × 106 cells were re-suspended in 50 μL normal saline, mixed with 50 μL Matrigel Matrix (1: 1), and inoculated into the subcutaneous tissue of NOD-SCID mice with 8 mice in each group. Two weeks later, the volume and size of the tumor were observed and recorded.
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
