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Peripheral blood was collected at 2 and 12 months of age by cardiac puncture. The blood was centrifuged at 2000 xg, and then serum was collected and stored at −20° C until further analyses. The concentrations of serum testosterone were measured by using ELISA kits (DRG Diagnostic, Springfield, NJ) with a reportable range of 0.06–25 ng/ml.
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