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Cardiac fibrosis was assessed using Masson Trichrome staining as previously described by us (Chu et al. 2011). 4‐μm thick sections of cardiac tissue were sectioned and for each section and measurement of fibrosis, 10 fields were chosen at random and imaged under light microscopy using the Olympus BH2 microscope at ×40 magnification. Fibrosis was then quantified using ImagePro Plus software (Adept Electronic Solutions Pty Ltd, Moorabin, Australia).
Copyright and License information: ©2016 The Authors. published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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