Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

MTT cellular viability assay

ML Maíra A. Lima
SS Suély V. da Silva
VF Vanessa M. Freitas
ask Ask a question
Favorite

After hormonal treatment for 24 h, the MTT assay was performed. In summary, cell culture supernatants were removed and a solution was added to each plate containing 100 μl of fresh medium DMEM F12 with 10 μl of an MTT solution (Calbiochem, Darmstadt, Germany) of 5 mg/mL in PBS, resulting in a final MTT concentration of 0.5 mg/ml. Cells were maintained in the incubator for 3 h until blue crystals were formed. At this point, DMSO (100 μl) was added to dissolve the crystals, the plate was homogenized, and absorbance reading was performed at 570 nm in a spectrophotometer.

Copyright and License information: Lima et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A