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CD34+ cells isolation
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In order to enrich samples in the progenitor and primitive fraction, we performed a positive selection using CD34 MicroBeads (Miltenyi Biotech), according to the manufacturer’s instructions. The CD34+ fraction was immediately used for functional assays, or cryopreserved for later use in freezing medium (DMEM 50%/HSA 40%/DMSO 10%). When available, 10,000 cells were used for purity assessment by flow cytometry.
Copyright and License information: ©2018 Ruiz et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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