Statistical analysis of clinical data was performed using the software package SPSS 19.0 software (IBM, Chicago, IL, United States). Chi-square test analyzed the association between B7-H3 or B3GALT4 and the clinicopathological data. The non-parametric Spearman test evaluated the correlation of B7-H3 and B3GALT4 expression. Overall survival (OS) was plotted using the Kaplan-Meier method and was compared using the log-rank test. Multivariate survival analysis was performed using the Cox proportional hazard model. All the in vitro experiments were performed in triplicate. A non-paired t-test analyzed differences in mean values between groups. All data were analyzed using GraphPad Prism 6.0 (GraphPad Software Inc., La Jolla, CA, United States). A P-value less than 0.05 indicated a statistically significant difference.
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