Neisseria gonorrhoeae antimicrobial susceptibility testing

The minimum inhibitory concentrations (MICs; mg/L) of penicillin G, ceftriaxone, tetracycline, spectinomycin, azithromycin and ciprofloxacin were analysed via the MIC method using agar dilution methodology on chocolate blood agar enriched with 1 % IsoVitaleX (Becton Dickinson, Sparks, MD, USA) according to the recommendations of the US Clinical and Laboratory Standards Institute [17].

For quality control, the N. gonorrhoeae ATCC 49226 reference strain was included twice in each test. All results were interpreted using whole MIC dilutions. Where available, breakpoints for susceptibility (S), intermediate susceptibility (I) and resistance (R) according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST; www.eucast.org) were used (Table 1).

Antimicrobial susceptibility of 124 Neisseria gonorrhoeae isolates from Russia in 2015

^a according to EUCAST (www.eucast.org)