Generation of iPSC lines

Somatic reprogramming was used to generate iPSC lines from peripheral blood mononuclear cells (PBMCs) of the patient with the KCNH2^T983I variant as confirmed by RT-PCR and from a healthy subject using the Sendai virus reprogramming protocol as described previously (11). iPSCs were also derived from an affected patient with a verified LQT2 (KCNH2^A561V) mutation (24) as described above. At least 3 colonies were generated from both patients and healthy control subject. All recruitment and consenting procedures conformed to the Stanford Institutional Review Board (IRB) approved protocol.