Knockdown of GPR41 in HepG2 cells

Gene silencing of GPR41 in HepG2 cells was performed as described previously [45]. Small interfering RNA (siRNA) against GPR41 (siGENOME Human FFAR3: GPR41 siRNA-1; and ON-TARGET plus Human FFAR3: GPR41 siRNA-2), and control siRNA (siGENOME Non-siRNA: control siRNA-1; and ON-TARGET plus Non-targeting: control siRNA-2) were purchased from GE Healthcare Dharmacon (Lafayette, CO, USA). HepG2 cells (70% confluence) were transfected with control siRNA or siRNA against GPR41 at a final concentration of 50 nmol/L, using a transfection reagent (DharmaFECT; Dharmacon). After a 72-h incubation, HepG2 cells were refreshed with DMEM and then treated for a further 24 h with cisplatin with or without NaP.