Complete Freund’s Adjuvant (CFA) model of inflammatory pain

Animals were allowed at least four days to habituate to the facility before the beginning of the study. Immediately following baseline assessment of mechanical thresholds, male C57Bl/6 mice, were lightly restrained and subcutaneously injected with CFA (5–10 μl, non-diluted) under the ventromedial surface of the left hindpaw. In electrophysiological and behavioral studies, sham mice were injected with an equal volume of saline to control for needle puncture and injectate volume.