hJBMMSCs were plated at 5 × 105 cells/well in a Flexcell biaxial 6-well plate overnight. The medium was replaced by fresh basal medium or OS 24 h before cells being subjected to sustained mechanical stretch (12% at 0.5 Hz) by the Flexcell FX-4000 tension system (Flexcell International Corp., USA). Cell layers were then collected for further analysis.
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