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Lentivirus-mediated shRNA silencing
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Endogenous CHTM1 was knockdown by the lentivirus-mediated shRNA approach [5]. The scramble shRNA construct was obtained from Addgene, Inc. (Cambridge, MA, USA). All CHTM1 specific shRNA constructs were obtained from Origene, MD, USA. Three different nucleotide sequences were used to target the human CHTM1: KD-1, 5′-CTTAAGGTAGTGACAGTCC-3′; KD-2, 5′-TCTGTCGAAGACACTCCTC-3′ and KD-3, 5′-TGGAAGTCCTGATATCCAG-3′. Addgene protocol was followed for virus production and infection [5].
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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